Animal Cognition

Object-based tasks are widely used in rodent behavioral research, yet object selection remains largely unsystematic. We present a paired-object validation protocol in which objects differ along one researcher-defined feature, allowing assessment of whether that feature is salient to the animal. Using six object pairs varying in height, color, shape, or aperture presence, we tested two wild-caught mice species with contrasting ecologies. Wood mice (Sylvaemus uralensis) and striped field mice (Apodemus agrarius) showed equal preference for both objects in most pairs, indicating that color, apertures, and apex shape differences are not salient under the tested conditions and can be used interchangeably in object recognition tasks. Height, however, produced ecology-predicted responses: arboreal wood mice avoided the shortest object while open-habitat striped field mice did not. These results demonstrate that the protocol successfully detects feature salience when present and that ecological background predicts which features matter. Summary StatementA systematic paired-object protocol reveals that most researcher-defined features (color, holes, shape) do not affect rodent exploration, but height preferences emerge in ecology-predicted patterns, demonstrating that feature salience is species-specific.

Traditionally, primates have been considered primarily visual animals. However, studies across a variety of taxa suggest that, in the context of food evaluation, the reliance on this sense might be more nuanced that previously thought, with dietary specialization and food item properties leading to differences in sensory prioritization. We performed a field-based study assessing the use of sensory cues during food evaluation as well as food-related behaviours such as muzzle contact in two mixed-sex groups of wild vervet monkeys including three age classes over a period of five months (nmonkeys = 44). Using a total of 18868 food evaluation observations collected over 44 hours of focal follows, we found that vervets mainly relied on their sense of vision when evaluating food (96.8% of all instances). Sensory usage varied according to food category and sex differences were only observed in the use of smell for a subset of these. Juveniles initiated muzzle contact and used tactile inspection more often than adults whereas females received muzzle contact more often than males. In addition, the low rejection rates suggest that most food items were familiar to the vervets regardless of age and sex. These findings are in line with optimal foraging theory according to which the food evaluation process should be adapted to the familiarity of food items and allows individuals to maximize their intake of energy and critical nutrients, while minimizing the time and effort in food evaluation.

The replicability of experimental results is considered a cornerstone of empirical research. However, the reproducibility of results from groups that have not undergone experimental manipulation -- the standard against which comparisons in an experiment are made -- has been almost entirely neglected in animal research. Our aim is to address this gap by exemplarily determining within-laboratory replicability using research in pigs, an increasingly popular large animal model species. Drawing on data from twelve independent porcine holeboard studies conducted in our laboratory, we examine the replicability of groups that were not subjected to experimental manipulation (typically the control group), eventually including groups on which the experimental treatments had no effect. These analyses were also performed on all eight studies involving the Terra x Finnish Landrace x Duroc pig breed, with all other breeds excluded to increase genetic uniformity. The holeboard is a complex spatial discrimination task in which an animal must learn to find food at four of sixteen possible locations (holes) arranged in a 4 x 4 matrix. The main variables measured are spatial working memory (WM), reference memory (RM) and the inter-visit interval (IVI), which serves as an index of motivation. All studies showed predominantly linear improvements in WM and learning rates across successive trial blocks. IVI showed greater variation across trialblocks, but this did not affect WM and RM learning, which are robust and replicable indices of spatial learning in pigs. Assessing replicability provides relevant information, such as whether behavioural and physiological traits in a model species are stably expressed and robust across studies. Including replicability research and publishing its results can stimulate the development and use of more replicable methods and workflows, thereby increasing scientific rigour. Provided the data are available and accessible, the next step should be to expand replicability studies to include those conducted in different laboratories.

Urbanisation is one of the most important forms of human-driven landscape change, altering wildlife populations in unprecedented ways. In terms of behaviour, for example, urbanisation is hypothesised to increase the likelihood of observing urban populations touching, exploring, and solving novel food-related tasks compared to rural areas. However, little is known about the impact of spatiotemporal patterns of urbanisation, particularly historical patterns of change, on these behaviours. We tested this in the worlds most urbanised carnivore, the red fox (Vulpes vulpes), by introducing novel food-related tasks (puzzle feeders) to 284 sites throughout Great Britain. We compared tactile and problem-solving behaviours in rural populations, recently colonised urban populations, and long-established urban populations (>40 years). Foxes from 27.4% of locations touched the tasks, foxes from 12.4% of locations solved them. Urban foxes were more likely to touch tasks compared to rural populations. Exploration time, exploratory diversity, and latency to touch tasks did not significantly differ across urban and rural locations. Urbanisation rate from 1994 to 2020 (26 years) did not significantly predict the likelihood of foxes touching or solving tasks across locations. Older urban populations - particularly from London - spent more time exploring tasks and displayed greater exploratory diversity and higher problem-solving success, despite more recent urban populations being equally likely to touch them. Collectively, our findings suggest that certain population characteristics, such as the likelihood of touching/engaging with novelty, potentially emerge early in urbanisation while other characteristics, such as greater exploratory and innovative behaviours, may emerge after long-term urban exposure across many decades. HighlightsO_LIHistorical impacts of urbanisation on wild animal behaviour are unclear. C_LIO_LIWe tested this with wild red foxes responses to novel food objects. C_LIO_LIUrban foxes were more likely to touch and exploit objects, especially from London. C_LIO_LIOlder urban foxes displayed more exploratory and innovative behaviours. C_LIO_LILength of urban exposure may help predict behavioural responses to novelty. C_LI

Social decisions often require animals to integrate information across multiple attributes of potential partners. Using biological motion stimuli, point-displays generated from tracked live shoals, we tested how adult zebrafish (Danio rerio) weigh shoal size and movement speed during social preference, and whether these preferences are susceptible to contextual manipulation by an asymmetrically placed alternative. In Experiment 1, we established a multi-attribute indifference point by presenting males and females with dichotomous contrasts in which shoal size and movement speed were traded off. Both sexes showed no preference when a larger, slower shoal (4 fish at 0.75x speed) was pitted against a smaller, faster shoal (2 fish at 1.25x speed), but preferred the smaller, faster shoal when the speed difference was greater (4 fish at 0.5x versus 2 fish at 1.25x), indicating that zebrafish are sensitive to graded differences in movement speed relative to numerical cues. In Experiment 2, unidimensional tests confirmed that both sexes preferred larger shoals when speed was held constant but revealed sex-based differences in speed sensitivity: males preferred faster-moving shoals at both shoal sizes tested, whereas females showed no significant speed preference. Male shoal size preferences were stronger at higher movement speeds, suggesting that speed modulates the strength of size preference. In Experiment 3, we tested the asymmetric dominance effect in males, the only sex sensitive to both dimensions, using the indifferent contrast from Experiment 1 as the primary options and four decoy shoals asymmetrically placed along either the size or speed dimension, under counterbalanced presentation orders. No decoy shifted male preference significantly from chance under any condition. These results indicate that zebrafish weigh social cues in a sex-specific manner, and that asymmetric decoy options do not induce preference biases in males when shoals vary along the dimensions of movement speed and size.

Human pointing is often used to test whether dogs extract object-specific information from human communicative cues. However, above-chance responses in standard object-choice tasks do not by themselves distinguish between a referential interpretation, in which the gesture identifies a specific target, and an attentional interpretation, in which it primarily biases behaviour toward a broader spatial region. We addressed this issue using an asymmetric six-cup arrangement designed to separate coarse side guidance from exact cup localisation more clearly than a symmetric multi-cup design. Performance in domestic dogs was analysed using three measures: the probability of reaching the correct side, the probability of choosing the correct cup overall, and the probability of choosing the correct cup conditional on having first reached the correct side. The principal comparison involved three matched trial classes: the symmetric 3-vs-3 condition, 2-vs-4 trials with the baited cup on the 2-cup side, and 2-vs-4 trials with the baited cup on the 4-cup side. Descriptively, pointing trials exceeded matched no-point control trials more clearly for side selection than for overall cup choice. The clearest condition effect was observed at the level of side guidance. Dogs were most likely to reach the correct side when the baited cup was located on the 4-cup side of the unequal arrangement. Mixed-effects models confirmed a reliable group effect for side accuracy, whereas overall cup accuracy showed only a weaker and less robust condition effect, and within-side localisation revealed no reliable group difference once condition-specific chance baselines were taken into account. A complementary generative model comparison converged on the same conclusion: a referential-only model fit poorly, an attention-only model captured most of the grouped outcome structure, and a combined model yielded only a modest improvement. Dog point-following is therefore best understood as a layered process dominated by attentional guidance, with only limited additional target-specific localisation.

The midsession reversal (MSR) task is frequently used to study behavioral flexibility and decision strategies in animals. In a typical version of the task, subjects complete 80 trials in which they choose between two simultaneously presented stimuli, S1 and S2. During the first 40 trials, responses to S1 are reinforced, whereas responses to S2 are not. The contingencies then reverse without warning: From trial 41 to 80, only responses to S2 are reinforced. In birds, performance in this task is often characterized by anticipatory and perseverative errors around the reversal point, suggesting a reliance on elapsed time since the session began. In contrast, rats tested in operant conditioning chambers typically show near-optimal performance with few errors, a pattern often interpreted as evidence that rats rely primarily on local reinforcement cues rather than temporal information. The present study investigated whether rats exclusively rely on local cues in the MSR task or whether temporal information also contributes to the decision process. Two groups of rats were trained with different intertrial intervals (ITIs; 5 s or 10 s) while the reversal point remained fixed at Trial 41. During acquisition, both groups diplayed similar learning rates and near-optimal steady-state performance with minimal anticipatory or perseverative errors. However, when the ITI was manipulated in probe sessions, systematic shifts in switching behavior emerged. Rats adjusted their choices according to the temporal midpoint experienced during training rather than the nominal trial number of the reversal. These results suggest that rats rely on a mixed strategy that integrates local reinforcement cues with global timing information. Temporal control may therefore be present even when it is not expressed during standard training conditions.

Judgment bias tasks are increasingly used to assess affective states in animals, yet the extent to which they might reflect transient states or stable traits remains unclear. Here, we tested bumblebees (Bombus terrestris) in an active choice task across three repeated sessions to assess individual consistency in the absence of any manipulation. Bees were trained to associate each of two colours with either a high or a low reward, presented in separate chambers. During testing, they were presented with ambiguous colours. Bees were more likely to choose the high reward chamber and to choose more quickly in response to colours closer to the positive colour. The latency to choose the cues showed significant and moderate repeatability across sessions, suggesting a stable, trait-like underlying component. In contrast, the repeatability of the chamber choices was negligible, indicating that such responses might be largely state-dependent and influenced by situational factors. These findings suggest that judgment biases, particularly as assessed through an active choice task reflect states affected by external factors. Active choice tasks may help disentangle stable behavioural traits from transient affective states in invertebrates.

Many animals use the Earths magnetic field as a directional reference, for long-distance, but also for local movements. Among those are amphibians which can be trained along the y-axis (the shore - deep gradient) in their aquatic environment. We used a light gradient (light-dark axis) to train larvae of the European green toad (Bufotes viridis) towards a magnetic direction, testing their magnetoreception ability. After training we tested the individual animals four times, in the four cardinal magnetic field directions (N, E, S, W). Recent developments in circular statistics allowed us to use the larvaes responses in a mixed effects models (the individual as the random factor) and tease the overall and individual responses apart. We used control simulations to test whether the mixed effects models could produce false positive findings, which confirmed the validity of this approach. Our results clearly show a trained magnetic compass response for the green toad, adding another animal to the list of magnetoreceptive animals. Interestingly, when analyzing just the first choice after release, there was only a magnetic effect. However, over the course of the entire 2 minutes trial, animals also showed untrained magnetic as well as non-magnetic responses, highlighting the complexity of small-scale animal orientation, with many interacting cues and motivations. Switching to repeated measures experimental design together with the newly developed circular statistical approaches can therefore be used to better understand the entirety of the animal orientation strategies, going beyond the overall effect. Our approach has the potential to study different aspects of animal orientation in the same experiment (i.e., magnetic alignment and trained magnetic effects) and therefore bridge the gaps between different lines of research.

Object play - seemingly non-functional interactions with objects - can promote the development of foraging skills, tool use, and behavioral innovation. Among Catarrhine monkeys, it was described in macaques and baboons. Wild geladas, although closely related to baboons, have been described as lacking object play (observed only in captivity) linked to their specialized grazing ecology. Here, we provide the first evidence of both social and solitary object play in a wild gelada population (NOMUs=13) at Debre Libanos (Ethiopia) and compare it with object play in sympatric olive baboons (Nindividuals=42). Notably, immature geladas engaged in object play both socially and solitarily, but the latter case was most frequent also with novel objects introduced by researchers. Solitary object play occurred at levels comparable to those of baboons, challenging previous reports of limited object interest in geladas. This finding aligns with the occurrence of object play in phylogenetically related species and with the retention in wild geladas of arboreal behavior and fruit consumption and hand morphology enhancing fine manipulation. Hence, object play in geladas under certain environmental conditions may reflect a biologically rooted capacity and underscores the importance of ecological variability, as distinct behavioral ecotypes can emerge across different populations of the same species.

Numerical abilities are widespread in the animal kingdom and are not exclusive to humans. Domestic chicks (Gallus gallus) have been shown to discriminate numerosities spontaneously, but prior research has focused exclusively on the visual modality. Whether chicks can discriminate numerical information in the auditory domain remains unknown, despite evidence that they can perceive other auditory features such as tone and rhythm. In this study, we investigated spontaneous numerical discrimination in the auditory modality in naive domestic chicks. In Experiment 1, newly-hatched chicks were tested for their ability to discriminate between two auditory sequences differing in numerosity (4 vs. 12 identical sounds), with and without controlling for continuous variables such as duration and total sound amount. Experiment 2 examined chicks filial imprinting responses to familiar or unfamiliar numerosities. Experiment 3 controlled for potential spontaneous preferences for a single longer sound versus a shorter one. Our results showed a preference for the 12-sound sequence only when duration and total sound amount were not matched. When these continuous variables were controlled, no spontaneous numerical preference emerged. Experiment 2 revealed an overall preference for the 12-sound sequence regardless of imprinting conditions, while Experiment 3 confirmed that chicks do not have an inherent preference for longer sounds. These findings suggest that chicks are sensitive to overall magnitude in the auditory domain but do not spontaneously discriminate numerical differences when other continuous variables are held constant. Future studies will explore how specific stimulus features, such as heterogeneity of sounds, influence these preferences.

Despite growing evidence that many animals can evaluate quantities, the ecological relevance of numerical cognition remains debated, particularly outside vertebrates. Would individuals still rely on numerousness if less computationally demanding cues, visual features extracted at the early stage of visual processing, were available to assess quantity? In primates, individuals show a numerical bias as they tend to rely on the number of items rather than non-numerical cues, such as total area, to categorize quantities. In this study, we trained free-flying honeybees to discriminate between two and four items in conditions where numerosity covaried with the total area and perimeter (Experiment Size) or the convex hull (Experiment Space) cues, mimicking ecological contexts. Transfer tests assessed which numerical or non-numerical cues were learned and preferentially used by the bees. Bees primarily relied on numerousness over these non-numerical cues. Individual analyses revealed two consistent strategies: a "numerical bias" strategy, in which bees encoded numerical information while ignoring non-numerical cues, and a "generalist" strategy, where bees flexibly switched between cues and favored non-numerical information when cues conflicted. We further reported improved discrimination when smaller quantities appeared on the left and larger ones on the right, consistent with an oriented mental number line. Together, these findings demonstrate a spontaneous numerical bias in honeybees and reveal that individuals within the same species can adopt distinct strategies when evaluating quantity. Our findings also suggest that distantly related taxa like bees and primates may have independently evolved comparable mechanisms for quantity evaluation.

Sentinel behaviour occurs when individuals use raised positions to scan for predators while the rest of the group forages. Here, we investigated whether a colonial cooperatively breeding species that forages in large groups, the sociable weaver, Philetairus socius, displays sentinel behaviour. This behaviour has been reported in species with similar ecology, behaviour and foraging habits, (e.g. ground foraging in open habitats where aerial predators are common) and, hence, we expected that it could occur in sociable weavers. On the other hand, sentinel behaviour appears to be less common in species that live in very large groups. We used an experimental set-up consisting of an artificial feeding station and perches to assess occurrence of sentinel related behaviours: (i) perching events > 30s on an elevated position, (ii) head-movements and (iii) alarm calling. Birds were seldom observed perching while others fed, and those that did, perched for periods that were too short to be considered as sentinel behaviour (less than 5s on average). Our results suggest that this behaviour is uncommon or even absent in sociable weavers. We discuss whether other factors such as foraging in very large groups, or interspecific foraging associations might make sentinel behaviour less important in this species.

The ability of animals to innovate - solve novel problems - can shape their ecology and evolution. Here we investigate how individual traits and environmental complexity relate to successful solving of a novel problem. We presented foraging bumble bees (Bombus impatiens) with artificial flowers of not-previously-encountered shapes and recorded the bees latency to access nectar. We measured individual foraging traits across multiple trips with simple flowers that did not require innovation, and bees were foraging either in a simple or complex environment (cluttered flight arena). Bees in complex environments took longer to find and were less likely to land on novel flowers, indicating that environmental complexity may take up cognitive resources and make search more difficult. However, we did not find an effect of environmental treatment on the ability or time to access reward in novel flowers once bees had landed on them. In contrast, behavioral traits significantly predicted how quickly bees solved novel flowers. In particular, overall foraging tempo as well as routine formation, i.e. how much bees followed a fixed route on known flowers, predicted innovation - faster bees innovated faster, and bees with more repetitive foraging sequences were slower to solve the novel tasks. Overall, while the degree of evolutionary novelty in tasks or solutions is always hard to evaluate, our findings demonstrate that environment and individual traits may affect innovation in different ways. Individuals in simple environments may be more likely to detect, and individuals that are generally faster and have a lower tendency to develop fixed routines may be more likely to solve, novel tasks.

When novel nutrient-rich food sources become available to species sharing the same natural habitat, interspecies competition may arise, yielding insights into the ecological and social dynamics of the observed species. Here, we investigated food consumption patterns, and consequent social interactions, by two sympatric species of mice in response to a novel nutrient-rich food source. By deploying, in the mices natural habitat, baited video-monitored chambers, we collected, over a 5-month period, 1805 observations of food visiting by Apodemus agrarius and Apodemus flavicollis. We also documented interspecific encounters, with 86.7% of the cases showing agonism. In these interspecies agonistic encounters, A. flavicollis was always the initiator of agonism, attacking within 2 sec in 92.3% of the cases, and being dominant over A. agrarius in 84.6%. Analysis of food visiting behavior revealed that, initially, both species preferred nocturnality. However, after the interspecies fights, A. agrarius switched its temporal preference to diurnality, leading to temporal niche segregation between the two species and a significant reduction of interspecies encounters. Moreover, A. agrarius demonstrated hour-specific avoidance of A. flavicollis, visiting significantly less in hours with A. flavicollis compared to hours without. Through temporal niche switching, A. agrarius managed to access the food source safely, without fights. In contrast, A. flavicollis remained consistently nocturnal across the entire study. Notably, our study presents the first 24h foraging actogram for free-living rodents. Moreover, while rodent interspecific competition is a well-known phenomenon, most of what we know about it comes from indirect observations. Direct observations of rodent interspecific interactions in nature are rare. Our work is the first direct (video-monitored) observation of temporal switch-inducing interspecies interactions in nature. As free-living rodents are currently considered a major model system for the study of interspecific competition, these results may offer precious insights for a better understanding of social dynamics, especially in asymmetric relationships. Furthermore, our findings highlight the significance of considering temporal dynamics in studies of interspecific interactions.

Parental care can shape post-fledging behaviour through provisioning, guidance and social information, yet its absence may alter how young birds establish space use and habitat preferences. We tested the consequences of absent parental care by comparing, hand-reared juvenile herring gulls released without parents with wild, parent-reared conspecifics, focusing on the first two months after fledging. Wild juveniles frequently revisited their natal nest during the first month, whereas hand-reared birds rarely returned to the release site; revisits declined in both groups by the second month but remained more common in wild birds. Wild juveniles used smaller ranges that subsequently expanded, while hand-reared birds began with larger ranges that later contracted, leading to convergence. Contrary to expectation, wild juveniles occurred in areas with higher human population density than hand-reared birds. Habitat use also differed between groups and changed over time. Early on, wild juveniles concentrated activity in anthropogenic and marine habitats, whereas hand-reared birds used rural green habitats more. Later, both groups shifted away from marine areas towards rural green habitats, reducing but not eliminating between-group differences. Short-term survival, did not differ between hand-reared and wild juveniles, indicating that parental care primarily reshaped early space use and habitat choice rather than immediate survival.

Species in stable social groups engage in diverse social interactions, where social competence - the ability to adjust behaviour using social information - can influence fitness. Yet, whether adaptive behavioural flexibility is expressed across contexts within individuals remains relatively untested. To address this, we exposed cooperatively breeding cichlids (Neolamprologus pulcher) to a role-reversal paradigm. In this species, the early social environment shapes social competence, with more competent individuals adjusting behaviour flexibly to social challenges, while individuals also show consistent differences in traits such as aggression. In the present study, individuals were successively assigned to two contrasting roles, smaller territory owners (TOs) and larger intruders (INTs). We predicted role-specific social competence metrics based on behaviours facilitating shelter acquisition. Social competence metrics correlated within, but not across the two roles. Competent TOs showed shorter latencies to submit, more submissive responses to received aggression, and low aggression after initial submission. Competent INTs escalated quickly and relied more on overt aggression rather than displays, allowing faster shelter acquisition. Across roles, individuals competent as TOs were not competent as INTs. In contrast, consistent individual differences in aggression across social roles suggest that stable behavioural tendencies ( animal personalities) may constrain how social competence shapes behavioural strategies.

Predation is a driving force in the ecology and evolution of prey, and primates exhibit diverse anti-predator strategies for minimizing risk. Because these behaviors can be costly, individuals must balance costs and benefits when responding to perceived threats. The cognitive capacity and behavioral plasticity of baboons make them an ideal taxon for studying the context-dependent variation in anti-predator strategies. Here, we used an autonomous, motion-activated playback experiment to study the behavioral responses of chacma baboons (Papio ursinus griseipes) to simulated predator encounters in Gorongosa National Park, Mozambique. We compared responses in 2021, when predator densities were relatively low, to responses in 2024, after predation increased due to lion (Panthera leo) population recovery and African wild dog (Lycaon pictus) reintroduction. We compared flight and vigilance responses to vocalizations of these common predators with responses to leopard (Panthera pardus), historically a key predator; spotted hyena (Crocuta crocuta), a rare predator; and cheetah (Acinonyx jubatus), absent historically and currently. We also assessed how responses varied with habitat, age-sex class, presence of offspring, and group size. Across 916 predator playbacks, baboons fled in 19% and displayed vigilance in 71% of trials. When predator density was higher, baboons displayed weakened antipredator responses, consistent with the risk allocation hypothesis. Baboons were more likely to flee in response to lion and wild dog cues. Juveniles fled more frequently than other demographic classes, while adult females with offspring were more vigilant. Overall, responses were highly heterogeneous, reflecting the substantial intraspecific variation and behavioral flexibility characteristic of baboons.

Humans order numbers in space from left to right, with smaller quantities represented preferentially in the left hemispace and larger ones in the right hemispace. The direction of this mental number line (MNL), or more generally of number-space associations (NSA), is influenced by cultural habits such as reading and writing direction. However, a growing body of evidence from pre-verbal infants and non-human animals suggests that number-space mappings may also have biological foundations. In non-human primates, evidence for a directional MNL remains mixed, partly due to small sample sizes and methodological heterogeneity. Here, we tested samples of rhesus (Macaca mulatta) and crab-eating macaques (Macaca fascicularis) across two experiments using spontaneous food-related tasks. In Experiment 1, monkeys chose between identical food quantities (1x1 to 24x24) presented on the left and right. No systematic spatial choice bias emerged as a function of numerical magnitude, and hand use did not differ across exact numerical pairs, although exploratory analyses revealed magnitude-related modulations of manual responses. In Experiment 2, monkeys were habituated to small (4x4) or large (16x16) quantities and subsequently tested with the alternative quantity. Result showed significantly more leftward choices following numerical decreases (16[->]4) and more rightward choices following numerical increases (4[->]16), indicating that relative numerical context, rather than absolute magnitude, elicited directional spatial biases. These findings suggest that in macaques, number-space associations emerge most robustly in comparative contexts involving expectancy violations of magnitude.

Animals must continually balance foraging with the risk of predation. In complex natural environments, this means quickly distinguishing between threats and harmless situations. We investigated how site-associated coral reef fishes decide to escape in response to visual cues mimicking predator attacks, using controlled underwater presentations of looming stimuli at varying speeds. We measured escape responses across species and social contexts, comparing them to predator attack speeds observed in the same habitat. Escape responses were highly sensitive to the speed of the looming stimulus, with no responses occurring at low speeds. The speeds triggering escape matched those of predator attacks, whereas cruising swim speeds never triggered a response. Species employed distinct antipredator strategies: Brown Chromis foraged away from shelter with high responsiveness, whereas Bicolor Damselfish remained shelter-dependent with lower escape propensities. Contrary to expectations, the social factors did not affect responses in this study. These findings demonstrate that reef fish are highly sensitive to the approach speed of objects, with species-specific strategies further shaping behaviors. By combining realistic visual threats with natural predator attack data, this study offers insight into how animals make escape decisions in complex, real-world environments.